Abstracts
Résumé
L’analyse à grande échelle des protéines, également appelée analyse protéomique, a parfois été présentée comme un eldorado de la biologie moderne. La quête de l’Eldorado a conduit nombre de conquistadors à s’attaquer à une jungle impénétrable qui fut leur tombeau. Il serait regrettable que les biologistes subissent le même sort, et cet article va s’employer à éclaircir la jungle de l’analyse protéomique. Les grands concepts de l’analyse protéomique seront présentés, ainsi que les technologies actuelles utilisées dans le domaine. Les performances comme les limitations de ces technologies seront exposées, afin de donner au lecteur une vue aussi raisonnée que possible de l’état de l’art dans ce domaine de recherche. Enfin, les grandes applications actuelles et les développements prévisibles de l’analyse protéomique en thérapeutique seront discutés.
Summary
The present paper aims at clarifying some important aspects of proteomics, i.e. the large scale analysis of proteins. To this purpose, the main types of proteomic analyses are presented, i.e. those aiming at determining expression levels and those aiming at unravelling protein-protein interactions networks. Their performances and limitations are outlined, as well as their potential applications in biomedicine, to give an reasoned view of the current state of the art.
Appendices
Références
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