Abstracts
Abstract
The susceptibility of adults and larvae of the Japanese beetle to isolates of the fungi Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Lecanicillium longisporum and L. muscarium (Ascomycota: Hypocreales) was evaluated in laboratory bioassays. The presence of intra- and interspecific variations regarding the pathogenicity of the isolates was investigated. Results show that most of the fungal isolates caused mortality in adults and larvae. Isolates M. anisopliae INRS 705 and B. bassiana INRS 236 induced 70.3% and 65.2% of mortality in P. japonica adults, respectively, and both caused the death of about 37% of larvae. Of the 17 tested isolates, 13 caused larval mortality. However, no significant difference was found between the susceptibility of larvae to isolates from different species and those from a same species. Lecanicillium species are undoubtedly not pathogenic to adults and seem to have few effects on larvae. Based on the results obtained with the selected isolates, intra- and interspecific differences relative to the pathogenicity of the isolates appeared to be present. Overall, this study expanded the knowledge about P. japonica susceptibility towards entomopathogenic Hypocreales fungi. The implications of this study regarding the development of a biological control agent are discussed.
Keywords:
- Beauveria bassiana,
- Japanese beetle,
- Lecanicillium longisporum,
- Lecanicillium muscarium,
- Metarhizium anisopliae,
- microbial control,
- Popillia japonica
Résumé
La vulnérabilité des adultes et des larves du scarabée japonais aux isolats des champignons Beauveria bassiana, B. brongniartii, Metarhizium anisopliae, Lecanicillium longisporum et L. muscarium (Ascomycota : Hypocreales) a été évaluée lors de tests biologiques en laboratoire. La présence de variations intra- et interspécifiques concernant le pouvoir pathogène des isolats a été étudiée. Les résultats montrent que la plupart des isolats fongiques provoquent de la mortalité chez les adultes et les larves. Les isolats M. anisopliae INRS 705 et B. bassiana INRS 236 ont mené à des taux de mortalité de 70,3 % et 65,2 % chez les adultes de P. japonica, respectivement, et les deux isolats ont provoqué la mort d’environ 37 % des larves. Des 17 isolats testés, 13 ont causé de la mortalité larvaire. Cependant, aucune différence importante n’a été notée entre la vulnérabilité des larves aux isolats de différentes espèces et à ceux de la même espèce. Les espèces Lecanicillium n’ont clairement pas de pouvoir pathogène chez les adultes et semblent n’avoir que peu d’effet chez les larves. En se fondant sur les résultats obtenus avec les isolats choisis, des différences intra- et interspécifiques concernant le pouvoir pathogène des isolats semblent être présentes. Dans son ensemble, l’étude a permis d’accroître les connaissances sur la vulnérabilité de P. japonica aux champignons pathogènes de l’ordre Hypocreales. Les conséquences de cette étude pour le développement d’un biopesticide sont présentées.
Mots-clés :
- Beauveria bassiana,
- Lecanicillium longisporum,
- Lecanicillium muscarium,
- lutte microbienne,
- Metarhizium anisopliae,
- Popillia japonica,
- scarabée japonais
Appendices
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