Abstracts
Abstract
This research was carried out to study the antifungal properties of Medicago sativa L. against phytopathogenic fungus Rhizoctonia solani J.G. Kühn. Methanolic extract of M. sativa was estimated in vitro against the R. solani. M. sativa methanolic extract was found to be highly efficient at the concentration of 5% as it reduced 74% test fungus biomass. Test plant was further partitioned by five organic fractions namely, n-butanol, chloroform, ethyl acetate, acetone and n-hexane. These isolated fractions and synthetic fungicide control group were evaluated in vitro against R. solani. Results showed that ethyl acetate fraction and fungicide were most effectively to repress the growth of R. solani up to 61-67%. This ethyl acetate fraction was further carried out for analysis of GC-MS and nine bioactive agents were found. The most copious compounds were phytol (RT: 20.84), 1,2,3-Propanetriol, monoacetate (RT: 12.47), Z,Z-3, 15-Octadecadien-1-olacetate (RT: 21.75), Hexadecanoic acid, ethyl ester (RT: 19.72) and Benzene, nitro- (RT: 10.20). The present study can be concluded that M. sativa possesses antifungal constituents against R. solani.
Keywords:
- bioassay,
- in vitro,
- GC-MS,
- methanolic extract
Résumé
Cette recherche a été menée afin d’étudier les propriétés antifongiques de Medicago sativa L. contre le champignon phytopathogène Rhizoctonia solani J.G. Kühn. L’extrait méthanolique de M. sativa contre R. solani a été évalué in vitro. L’extrait méthanolique de M. sativa s’est avéré très efficace à une concentration de 5%, car il réduisait de 74% la biomasse du champignon à l’essai. L’extrait de plante a ensuite été divisé en cinq fractions organiques à savoir le n-butanol, le chloroforme, l’acétate d’éthyle, l’acétone et le n-hexane. Ces fractions isolées et le groupe témoin de fongicide synthétique ont été évalués in vitro contre R. solani. Les résultats ont montré que la fraction d’acétate d’éthyle et le fongicide étaient les plus efficaces pour réprimer la croissance de R. solani jusqu’à 61-67%. Cette fraction d’acétate d’éthyle a ensuite été analysée par CPG-SM et neuf agents bioactifs ont été trouvés. Les composés les plus abondants étaient le phytol (TR : 20,84), le 1,2,3-Propanetriol, monoacétate (TR : 12,47), Z,Z-3, 15-Octadécadien-1-olacétate (TR : 21,75), l’acide hexadécanoïque, ester (TR : 19,72) et le benzène, nitro- (TR : 10,20). La présente étude peut être conclue que M. sativa possède des constituants antifongiques contre R. solani.
Mots-clés :
- bioessai,
- in vitro,
- CPG-MS,
- extrait méthanolique
Appendices
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