Résumés
Résumé
La localisation intracellulaire des ARN est, dans de nombreuses espèces, impliquée dans les phénomènes de polarité cellulaire. Le plus souvent, l’ARN se localise en fonction d’une polarité préexistante et n’influence pas cette asymétrie. Cependant, dans le cas de l’actine β des vertébrés, le transport et la localisation de cet ARN sont directement impliqués dans l’établissement et le maintien de la polarisation cellulaire. Cela est vrai dans des cellules fibroblastiques en déplacement, mais aussi lors de la croissance axonale des neurones immatures. De manière remarquable, une altération du transport de cet ARN pourrait être liée à l’apparition de maladies chez l’homme. Cet article décrit l’état actuel des connaissances sur le rôle et les mécanismes de la localisation de l’ARNm de l’actine β.
Summary
In many species, intracellular mRNA localization is linked to cell polarity. In many cases however, mRNAs become localized as a result of a pre-existing cell-polarity, and they do not modify it. Remarkably, in the case β actin mRNA in vertebrate, it has been shown that the transport and localization of this RNA is required for the establishment and maintenance of cell polarity. This occurs in fibroblasts, but, very interestingly, in immature neurons as well. This review will describe the functions and mechanisms of actin mRNA localization.
Parties annexes
Références
- 1. Prodon F, Prulière G, Chenevert J, Sardet C. Établissement et expression des axes embryonnaires : comparaison entre différents organismes modèles. Med Sci (Paris) 2004 ; 20 : 526-38.
- 2. Bouget F, Gerttula S, Shaw S, Quatrano R. Localization of actin mRNA during the establishment of cell polarity and early cell divisions in Fucus embryos. Plant Cell 1996 ; 8 : 189-201.
- 3. Lawrence J, Singer R. Intracellular localization of messenger RNAs for cytoskeletal proteins. Cell 1986 ; 45 : 407-15.
- 4. Kislauskis E, Li Z, Singer R, Taneja K. Isoform-specific 3’-untranslated sequences sort alpha-cardiac and beta-cytoplasmic actin messenger RNAs to different cytoplasmic compartments. J Cell Biol 1993 ; 123 : 165-72.
- 5. Kislauskis E, Zhu X, Singer R. Sequences responsible for intracellular localization of beta-actin messenger RNA also affect cell phenotype. J Cell Biol 1994 ; 127 : 441-51.
- 6. Kislauskis E, Zhu X, Singer R. Beta-actin messenger RNA localization and protein synthesis augment cell motility. J Cell Biol 1997 ; 136 : 1263-70.
- 7. Shestakova E, Singer R, Condeelis J. The physiological significance of beta-actin mRNA localization in determining cell polarity and directional motility. Proc Natl Acad Sci USA 2001 ; 98 : 7045-50.
- 8. Shestakova E, Wyckoff J, Jones J, et al. Correlation of beta-actin messenger RNA localization with metastatic potential in rat adenocarcinoma cell lines. Cancer Res 1999 ; 59 : 1202-5.
- 9. Sundell C, Singer R. Requirement of microfilaments in sorting of actin messenger RNA. Science 1991 ; 253 : 1275-7.
- 10. Bashirullah A, Cooperstock RL, Lipshitz HD. RNA localization in development. Annu Rev Biochem 1998 ; 67 : 335-94.
- 11. Groisman I, Huang Y, Mendez R, et al. CPEB, maskin, and cyclin B1 mRNA at the mitotic apparatus : Implications for local translational control of cell division. Cell 2000 ; 103 : 435-47.
- 12. Lambert J, Nagy L. Asymmetric inheritance of centrosomally localized mRNAs during embryonic cleavages. Nature 2002 ; 420 : 682-6.
- 13. Deshler J, Highett M, Schnapp B. Localization of Xenopus Vg1 mRNA by Vera protein and the endoplasmic reticulum. Science 1997 ; 276 : 1128-31.
- 14. Jankovics F, Sinka R, Erdelyi M. An interaction type of genetic screen reveals a role of the Rab11 gene in oskar mRNA localization in the developing Drosophila melanogaster oocyte. Genetics 2001 ; 158 : 1177-88.
- 15. Basyuk E, Galli T, Mougel M, et al. Retroviral genomic RNAs are transported to the plasma membrane by endosomal vesicles. Dev Cell 2003 ; 5 : 161-74.
- 16. Bertrand E, Chartrand P, Schaefer M, et al. Localization of ASH1 mRNA particles in living yeast. Mol Cell 1998 ; 2 : 437-45.
- 17. Li P, Yang X, Wasser M, et al. Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions. Cell 1997 ; 90 : 437-47.
- 18. Knowles R, Sabry J, Martone M, et al. Translocation of RNA granules in living neurons. J Neurosci 1996 ; 16 : 7812-20.
- 19. Ainger K, Avossa D, Morgan F, et al. Transport and localization of exogenous myelin basic protein mRNA microinjected into oligodendrocytes. J Cell Biol 1993 ; 123 : 431-41.
- 20. Barbarese E, Koppel D, Deutscher M, et al. Protein translation components are colocalized in granules in oligodendrocytes. J Cell Sci 1995 ; 108 : 2781-90.
- 21. Taneja KL, Lifshitz LM, Fay FS, Singer RH. Poly(A) RNA codistribution with microfilaments : Evaluation by in situ hybridization and quantitative digital imaging microscopy. J Cell Biol 1992 ; 119 : 1245-60.
- 22. Bassell G, Powers C, Taneja K, Singer R. Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts. J Cell Biol 1994 ; 126 : 863-76.
- 23. Bassell G, Singer R, Kosik K. Association of poly(A) mRNA with microtubules in cultured neurons. Neuron 1994 ; 12 : 571-82.
- 24. Luby-Phelps K, Castle P, Taylor D, Lanni F. Hindered diffusion of inert tracer particles in the cytoplasm of mouse 3T3 cells. Proc Natl Acad Sci USA 1987 ; 84 : 4910-3.
- 25. Yamada S, Wirtz D, Kuo SC. Mechanics of living cells measured by laser tracking microrheology. Biophys J 2000 ; 78 : 1736-47.
- 26. Fusco D, Accornero N, Lavoie B, et al. Single mRNA molecules demonstrate probabilistic movement in living Mammalian cells. Curr Biol 2003 ; 13 : 161-7.
- 27. Bassell G, Zhang H, Byrd A, et al. Sorting of beta-actin mRNA and protein to neurites and growth cones in culture. J Neurosci 1998 ; 18 : 251-65.
- 28. Ross A, Oleynikov Y, Kislauskis E, et al. Characterization of a beta-actin mRNA zipcode-binding protein. Mol Cell Biol 1997 ; 17 : 2158-65.
- 29. Havin L, Git A, Elisha Z, et al. RNA-binding protein conserved in both microtubule- and microfilament-based RNA localization. Genes Dev 1998 ; 12 : 1593-8.
- 30. Deshler J, Highett M, Abramson T, Schnapp B. A highly conserved RNA-binding protein for cytoplasmic mRNA localization in vertebrates. Curr Biol 1998 ; 8 : 489-96.
- 31. Oleynikov Y, Singer R. Real-time visualization of ZBP1 association with beta-actin mRNA during transcription and localization. Curr Biol 2003 ; 13 : 199-207.
- 32. Zhang H, Eom T, Oleynikov Y, et al. Neurotrophin-induced transport of a beta-actin mRNP complex increases beta-actin levels and stimulates growth cone motility. Neuron 2001 ; 31 : 261-75.
- 33. Gu W, Pan F, Zhang H, et al. A predominantly nuclear protein affecting cytoplasmic localization of beta-actin mRNA in fibroblasts and neurons. J Cell Biol 2002 ; 156 : 41-51.
- 34. Richter J, Lorenz L. Selective translation of mRNAs at synapses. Curr Opin Neurobiol 2002 ; 12 : 300-4.
- 35. Tiruchinapalli D, Oleynikov Y, Kelic S, et al. Activity-dependent trafficking and dynamic localization of zipcode binding protein 1 and beta-actin mRNA in dendrites and spines of hippocampal neurons. J Neurosci 2003 ; 23 : 3251-61.
- 36. Rossoll W, Jablonka S, Andreassi C, et al. Smn, the spinal muscular atrophy-determining gene product, modulates axon growth and localization of beta-actin mRNA in growth cones of motoneurons. J Cell Biol 2003 ; 163 : 801-12.