Résumés
Abstract
Light and transmission electron microscopy observations of Verticillium-inoculated eggplants are reported. Results of tests for cellulose and chitin are also presented. Opaque, vessel wall lining matter (VWLM) generally occurred, connected to elements identified as fungal cells, and extending into vessel secondary walls and across pits. VWLM was also confluent with similar matter extensively pervading middle lamellae and causing their alterations. The thickest VWLM was stratified as alternating opaque and more lucent bands, with the opaque ones being similar to the first layer deposited. Many loops were included in or bulged from the VWLM layers. Lace-like structures also occurred in vessel lumina as apparent distensions of the basic, first opaque layer of this VWLM. Large amounts of similar opaque matter occurred in the periphery of paratracheal cells with altered content. This matter contained filamentous-like structures and frequently opaque particles reminiscent of ribosomes. The pitted areas were much altered as an internal shred-like network, unlabelled for cellulose, covered by an outer opaque and compact cellulose-labelled layer, itself overlaid by an unlabelled VWLM. Release of labelled material from these altered areas only occurred at locations of VWLM ruptures. Detachment and alterations of the adjacent vessel wall secondary thickenings were noticeable and accounted for the presence of lucent cellulose-labelled matter in vessel lumina.
Keywords:
- Cell wall alterations,
- labelling for cellulose and chitin,
- opaque matter,
- periplasmic areas,
- verticillium wilt,
- vessel wall coating,
- vessel wall lining material
Résumé
Un objectif de cette étude a été de caractériser, dans des plants inoculés, les composantes exogènes aux éléments de vaisseaux, dont les enduits sur la paroi vasculaire, et leurs liens avec des altérations cellulaires et la présence de matière opaque dans les lamelles mitoyennes et le périplasme de cellules paratrachéales. Des tests pour localiser certains composés pariétaux ont été utilisés. Les enduits étaient formés de diverses couches, dont une première, mince et opaque et d’aspect membranaire, et d’autres plus épaisses et translucides alternant avec des couches opaques. Plusieurs gonflements circulaires se trouvaient dans ou à la marge de ces strates (VWLM). D’autres réseaux sinueux, apparemment issus de l’espacement des bandes formant la première couche de l’enduit, se trouvaient dans les vaisseaux. La matière périplasmique contenait des structures filamenteuses et de nombreuses particules opaques d’allure ribosomique. La partie interne des champs de ponctuations montrait des bandes opaques éparses, non marquées pour la cellulose, recouverte d’une couche plus compacte, marquée pour ce substrat. Les résidus altérés n’étaient libérés dans les vaisseaux qu’en cas de rupture du VWLM montrant l’absence de marquage pour ces deux substrats. Les épaississements secondaires des parois vasculaires étaient souvent altérés ou détachés de leur base, expliquant la présence de matière translucide marquée pour la cellulose dans les vaisseaux.
Mots clés:
- Bris parois cellulaires,
- enduits sur la paroi vasculaire,
- espaces périplasmiques,
- marquage pour cellulose et chitine,
- matière opaque,
- recouvrement des parois vasculaires,
- verticilliose
Parties annexes
References
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