Résumés
Abstract
Test tube plantlets were used to assess the transmission efficiency of Potato virus Y (PVY) from and to potato plants by the potato colonizing species green peach aphid (Myzus persicae) and the non-potato colonizing species soybean aphid (Aphis glycines). Similar levels of transmission of PVY by M. persicae were observed in the test tube plantlets and potted plants, demonstrating the reliability of this test for PVY transmission efficiency assay for aphids. The assay was then used to assess the transmission of PVYO and PVYN:O by M. persicae and A. glycines with two virus acquisition regimes, one with 5-min continuous probing and the other with 1-h acquisition access. The M. persicae mediated-transmission rate was 24.1% and 51.7% for PVYO and PVYN:O, respectively, under the 5-min acquisition regime; under the same acquisition regime, A. glycines led to 0.0% and 1.7% infection rates for PVYO and PVYN:O, respectively. Under the 1-h acquisition regime, no infection was observed except for PVYN:O by M. persicae, which exhibited an infection rate of 3.4%.
Keywords:
- Aphis glycines,
- Myzus persicae,
- plantlets,
- potato,
- Potato virus Y,
- test tube,
- transmission
Résumé
Des vitroplants ont été utilisés pour évaluer le taux de transmission du virus Y de la pomme de terre (PVY) entre plants de pomme de terre par un puceron inféodé à la pomme de terre, le puceron vert du pêcher (Myzus persicae), et un puceron non-inféodé à la pomme de terre, le puceron du soya (Aphis glycines). Des taux similaires d’efficacité de transmission du PVY par M. persicae ont été observés lorsque des vitroplants ou des plantes en pot étaient utilisés, ce qui démontre la validité de l’utilisation de vitroplants pour l’évaluation du taux de transmission du PVY par les pucerons. La même approche a par la suite été utilisée pour mesurer le taux de transmission du PVYO et du PVYN:O par M. persicae et par A. glycines sous deux régimes d’acquisition, soit 5 min de sondage continu et 1 h d’accès. Le taux de transmission de M. persicae a été de 24,1 % et de 51,7 % pour PVYO et PVYN:O, respectivement, sous un régime d’acquisition de 5 min, et de 0,0 % et 1,7 % pour PVYO et PVYN:O, respectivement, avec A. glycines. Sous un régime d’acquisition d’une heure, aucune infection n’a été observée à l’exception de PVYN:O par M. persicae qui a présenté un taux d’infection de 3,4 %.
Mots clés:
- Aphis glycines,
- Myzus persicae,
- vitroplants,
- virus Y de la pomme de terre,
- transmission
Parties annexes
References
- Baldauf, P.M., S.M. Gray, and K.L. Perry. 2006. Biological and serological properties of Potato virus Y isolates in northeastern United States potato. Plant Dis. 90 : 559-566.
- Basky, Z., and A. Almási. 2005. Differences in aphid transmissibility and translocation between PVYN and PVYO isolates. J. Pest Sci. 78 : 67-78.
- Bradley, R.H.E. 1954. Studies of the mechanism of transmission of potato virus Y by the green peach aphid, Myzus persicae (Sulz.) (Homoptera: Aphididae). Can. J. Zool. 32 : 64-73.
- Bradley, R.H.E., and D.W. Rideout. 1953. Comparative transmission of potato virus Y by four aphid species that infest potato. Can. J. Zool. 31 : 333-341.
- Crosslin, J.M., P.B. Hamm, D.C. Hane, J. Jaeger, C.R. Brown, P.J. Shiel, P.H. Berger, and R.E. Thornton. 2006. The occurrence of PVYO, PVYN, and PVYN: O strains of Potato virus Y in certified potato seed lot trials in Washington and Oregon. Plant Dis. 90 : 1102-1105.
- Davis, J.A., and E.B. Radcliffe. 2008. The importance of an invasive aphid species in vectoring a persistently transmitted potato virus: Aphis glycines is a vector of Potato leafroll virus. Plant Dis. 92 : 1515-1523.
- Davis, J.A., E.B. Radcliffe, and D.W. Ragsdale. 2005. Soybean aphid, Aphis glycines Matsumura, a new vector of Potato Virus Y in potato. Am. J. Potato Res. 81 : 101-105.
- Gibson, R.W., R.W. Payne, and N. Katis. 1988. The transmission of potato virus Y by aphids of different vectoring abilities. Ann. Appl. Biol. 113 : 35-43.
- Hane, D.C., and P. B. Hamm. 1999. Effects of seed borne potato virus Y infection in two potato cultivars expressing mild disease symptoms. Plant Dis. 83 : 43-45.
- Karasev, A.V., O.V. Nikolaeva, X. Hu, Z. Sielaff, J. Whitworth, J.H. Lorenzen, and S.M. Gray. 2010. Serological properties of ordinary and necrotic isolates of Potato virus Y: A case study of PVYN misidentification. Am. J. Potato Res. 87 : 1-9.
- Katis, N., and R.W. Gibson. 1985. Transmission of potato virus Y by cereal aphids. Potato Res. 28 : 65-70.
- Kotzampigikis, A., D. Hristova, and E. Tasheva-Terzieva. 2009. Virus-vector relationship between potato virus Y - PVY and Myzus persicae Sulzer. Bulg. J. Agric. Sci. 15 : 557-565.
- Lorenzen, J., P. Nolte, D. Martin, J.S. Pasche, and N.C. Gudmestad. 2008. NE-11 represents a new strain variant class of Potato virus Y. Arch. Virol. 153 : 517-525.
- Moreno, A., E. Hebrard, M. Uzest, S. Blanc, and A. Fereres. 2005. A single amino acid position in the helper component of cauliflower mosaic virus can change the spectrum of transmitting vector species. J. Virol. 79 : 13587-13593.
- Murashige, T., and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15 : 473-497.
- Nie, X., and R.P. Singh. 2001a. A novel usage of random primers for multiplex RT-PCR detection of virus and viroid in aphids, leaves, and tubers. J. Virol. Methods 91 : 37-49.
- Nie, X., and R.P. Singh. 2001b. Differential accumulation of Potato virus A and expression of pathogenesis-related genes in resistant potato cv. Shepody upon graft inoculation. Phytopathology 91 : 197-203.
- Nie, X., R.P. Singh, and M. Singh. 2004. Molecular and pathological characterization of N:O isolates of the Potato virus Y from Manitoba, Canada. Can. J. Plant Pathol. 26 : 573-583.
- Nie, B., M. Singh, A. Sullivan, R.P. Singh, C. Xie, and X. Nie. 2011. Recognition and molecular discrimination of severe and mild PVYO isolates of Potato virus Y in potatoes in New Brunswick, Canada. Plant Dis. 95 : 113-119.
- Nolte, P. 1997. Why a significant increase in virus and methods of control? Pages 24-26 in Proc. 16th Annual National Potato Council Seed Seminar, 4-6 Dec. 1997, Traverse City, MI, USA.
- Pelletier, Y., X. Nie, M. McClure, S. Whitney, and M.-A. Giguère. 2008. Behavior of bird cherry-oat aphid and green peach aphid in relation to Potato virus Y transmission. J. Econ. Entomol. 101 : 728-735.
- Piche, L.M., R.P. Singh, X. Nie, and N.C. Gudmestad. 2004. Diversity among Potato virus Y isolates obtained from potatoes grown in the United States. Phytopathology 94 : 1368-1375.
- Radcliffe, E.B., and D.W. Ragsdale. 2002. Invited Review. Aphid transmitted potato viruses: the importance of understanding vector biology. Am. J. Potato Res. 79 : 353-386.
- Ragsdale, D.W., D.A. Landis, J. Brodeur, G.E. Heimpel, and N. Desneux. 2011. Ecology and management of soybean aphid in North America. Annu. Rev. Entomol. 56 : 375-399.
- Shukla, D.D., C.W. Ward, and A.A. Brunt. 1994. The Potyviridae. CAB International, Wallingford, UK. 516 p.
- Singh, R.P., D.L. McLaren, X. Nie, and M. Singh. 2003. Possible escape of a recombinant isolate of Potato virus Y by serological indexing and methods of its detection. Plant Dis. 87 : 679-685.
- Uzest, M., D. Gargani, A. Dombrovsky, C. Cazevieille, D. Cot, and S. Blanc. 2010. The “acrostyle”: A newly described anatomical structure in aphid stylets. Arthropod Struct. Dev. 39 : 221-229.
- Verbeek, M., P.G.M. Piron, A.M. Dullemans, C. Cuperus, and R.A.A. Van Der Vlugt. 2009. Determination of aphid transmission efficiencies for N, NTN and Wilga strains of Potato virus Y. Ann. Appl. Biol. 156 : 39-49