McGill University Thèses et mémoires publiés en 1977

Two arginine-specific periplasmic binding proteins have been purified from E. coli ML-30 by affinity chromatography on an arginine-BE-Sepharose 48 gel. By this means it was possible to separate the arginine-binding activity of crude osmotic shock fluid into arginine specific (adsorbed) and arginine non-specific (non-adsorbed) binding frictions. The adsorbed fraction which was displaced with arginine was resolved into two arginine-specific binding proteins by gradient elution with arginine. Both proteins differ on the basis of affinities for arginine, Isoelectric Points, mobilities on anionic polyacryilimide gels, but have identical molecular weights, and similar amino acid composition. One of the binding protein, is similar …  Read more

Robertson, Dr. A. A.  Read more

A unit of study on the life and works of Galileo describes an attempt by a group of high school students to reproduce some of Galileos most famous experiments in mechanics and astronomy.  Read more

Dans le cadre d'un projet d'étude des maladies génétiques du tissu conjonctif chez l'homme, nous avons mis au point une technique d'analyse de la structure primaire du collagène. Nous ayons soumis des échantillons d'un mg des collagènes humains de type I, II, et III ainsi que des chaines a1(I) et a2 à une p digestions par la clôstridiopeptidase A. Les petits peptides ainsi obtenus ont été analysés par chromatographie en phase liquide à haute pression. Les profils d'élution obtenus forment des cartes peptidiques spécifiques qui sont liées a la structure primaire de chaque type de collagène étudié. […]  Read more

Le développement embryologique et les mécanismes croissance du squelette périphérique du mammifère sont révisés. On y revoit également les techniques d'évaluation de la nasse osseuse. On y discute la technique de l'autoradiographies et son application à l'étude de la cybernétique cellulaire et de la migration des cellules. […]  Read more

Bacterial luciferase, a heteropolymeric protein (alpha,beta) catalyzes the conversion of chemical energy into light. Information about the mechanism of bioluminescence can be drawn from the elucidation of amino acid residues involved in the active site of luciferase. It has been shown that the histidyl residues of luciferase can be specifically modified at pH 6.1 with ethoxyformic anhydride with a concomitant loss of bioluminescent activity. The modification of one histidine on the alpha subunit and two on the beta subunit completely inactivates the enzyme; however, only the histidyl residue that is modified on the a subunit of luciferase is essential for …  Read more

The transport of tryptophan (TRP), precursor of 5-hydroxytryptamine, (5-HT or serotonin) in the central nervous system, was investigated in brain areas known to vary in their number of serotoninergic cell bodies and nerve endings. The regional transport of TRP at the blood-brain barrier was studied with the Oldendorf technique. The passage of TRP across the cell membrane of neurones and glia was studied in slices prepared with a McIlwain tissue chopper, and with isolated nerve endings (synaptosomes) partially purified on density gradients. TRP accumulation was also measured in synaptosomes obtained from brains whose 5-HT nerve endings had been previously destroyed …  Read more